In this review, we discuss the prescription, composition, formulation, and mechanism of traditional Chinese medicine monomer, traditional Chinese medicine monomer complex, single herbs, and traditional Chinese patent medicine in the treatment of asthma. Hence, it is urgent to find new ideas for asthma prevention and treatment. At present, a subset of patients is resistant to the drug therapy for asthma. It has been verified that climate change can induce asthma in predisposed individuals and that atmospheric pollution can exacerbate asthma severity. In recent years, continuous industrial development has seriously impacted the climate and air quality at a global scale. Conclusion: The outcome of this study underscores the anti-inflammatory potential of Bio-Clean II in the treatment of bacterial inflammatory diseases.Īsthma is a complex syndrome with polygenetic tendency and multiple phenotypes, which has variable expiratory airflow limitation and respiratory symptoms that vary over time and in intensity. Meanwhile, the corticosterone level of the 7 days Bio-clean treated rats (9.40☑.30ng/mL) was found to be non-significantly lower (p=0.812) in comparison to the inflammation control (13.50☒.50ng/mL) while that of the 14 days Bio-clean II treated rats (6.80☑.00ng/mL) was significantly lower (p=0.026). Similarly, the serum level of anti-phospholipid antibodies of the 7 days (6.40☐.67 Uml) and 14 days (4.27☐.66 U/ml) Bio-Clean II treated rats was found to be significantly lower (p=0.02 and p=0.008, respectively) when compared to the inflammation control group (16.47☑.53 Uml). Results: The outcome of this results show that the serum level of C-reactive protein of the 7 days (1.05☐.06ng/ml) and 14 days (0.93☐.05ng/ml) Bio-Clean II treated rats was found to be significantly lower (p=0.002 and p=0.000, respectively) when compared to the inflammation control group (1.70☐.07ng/ml). Data generated were subjected to Statistical Package for Social Scientists-Version 20 (SPSS-20). The serum levels of C-reactive protein, corticosterone and antiphospholipid antibodies were assayed using ELISA kits, supplied by Elabscience Biotechnology Inc, USA. Serum was obtained from the clotted blood by centrifugation. After which, the rats were killed and a cardiac blood specimen was taken from each rat and transferred to plain bottles to clot. While rats in group 5 and group 6 were treated orally with the herbal remedy “Bio-Clean II” for 7 days and 14 days, respectively. Group 3 which served as the negative control received sterile phosphate buffer saline (PBS). Group 2 which served as the positive control received 50 mg diclofenac/kg and 500 mg ciprofloxacin/kg (positive control) in place of the Bio-Clean. Group 1 served as the inflammation control. Louis, USA), administered through intraperitoneal route using 1ml sterile needle and syringe, except for group 4 which served as the zero control (given water and food only throughout the experiment). Group 1, 2, 3, 5 and 6 were induced with a single dose of 5mg/Kg of purified LPS® (E.coli 0127:B8, Sigma-Aldrich, St. Materials and Methods: A total of 36 male Wistar rats weighing 150g±50g (mean±SD) were purchased and randomly assigned to six (6) groups of 6 rats each. Aim: The aim of this study is to assess the effects of Bio-Clean II on the C-reactive protein (CRP), corticosterone (CORT) and anti-phospholipid antibodies (aPLs) levels in rats exposed to bacterial lipopolysaccharide (LPS) using animal model. The precision in determining true values of the corticosterone level is low for these commercial ELISA kits, although they may be used to determine relative differences within studies.īackground: Bio-Clean II has been previously shown to fight viral infection, boost immunity, and possesses anti-inflammatory properties by regulating the serum level of inflammatory cytokines, as well as T-Helper 4 and Cytotoxic T-Lymphocytes in rats exposed to purified bacterial lipopolysaccharide (LPS). In conclusion, the commercial ELISA kits tested in the present experiment yielded different values of total corticosterone in the same serum samples. Overall the correlations between kits were high. There were no significant differences between the DRG-5186 and Enzo kits. Both the Arbor Assays and the DRG-4164 kits were significantly higher than the DRG-5186 and the Enzo kits. Trunk blood was sampled from 32 male Wistar rats 30 minutes after this mild stress exposure and analysed with each of four commercial ELISA kits. The aim of this experiment was simply to evaluate if four different and widely used commercial ELISA assays would yield the same or similar values of corticosterone in serum samples taken from laboratory rats after the mild stress of being held for sampling blood from the saphenous vein. Enzyme-linked immunosorbent assay (ELISA) kits are widely used to quantify corticosterone levels for the assessment of stress in laboratory animals.
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